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Antibodies sure to the blocking peptide no more bind on the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody alone, you can see which staining is particular.
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to be sure the very best results are...
This tutorial outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody.
Target Information Cyclin E belongs for the very conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of every mitotic event. Cyclin E sorts a posh with and functions to be a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is particularly degraded as cells development by way of S stage.
Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which here participates in cell-cycle regulated histone gene expression and performs a vital job in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two supplemental splice variants had been documented but thorough nucleotide sequence information is just not nevertheless out there.
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